Verification of receptor knockdown was done by creating a c-Met-6-Myc tagged gene product using the Gateway cloning system (Invitrogen)
Early ultrastructural studies showed alterations in the dermis of these animals, with loosely packed, thin, and twisted ribbon-like collagen fibrils that displayed a typical hieroglyphic aspect on cross-sections, pointing toward impaired collagen biosynthesis and fibrillogenesis
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The proteins were visualized by exposure machine (GE, Amersham Imager 680) using Pierce ECL Plus Western Blotting Substrate (Thermo Fisher Scientific, 32132) and the protein bands were quantitatively analyzed with ImageJ software
